Recovery of Biomolecules using Green Solvents
With the increasing interest of Biotechnology, and to keep a green and sustainable development of industry, the recovery of biomolecules is a critical step. In Chemical Engineering, separation has always accounted for a significant part of the process costs. In Biotechnology, separation is even more difficult (and expensive) due to the nature of the compounds in the mixtures. The goal is to apply Aqueous Two-Phase Systems (ATPS) for the recovery of different target solutes, namely amino acids, proteins and enzymes that are of interest for different biotech and pharma units. The focus is on both conventional formulations (two polymers or a polymer and a salt) and new biphasic systems, with ionic liquids and/or deep eutectic solvents.
S.C. Silvério, J. Gracia, J.A. Teixeira, E.A. Macedo. Polyethylene glycol 8000+ citrate salts aqueous two-phase systems: Relative hydrophobicity of the equilibrium phases. Fluid Phase Equilibria, 407, 298-303, 2016
K. Wysoczanska, S.C. Silvério, J.A. Teixeira, E.A. Macedo. Cation effect on the (PEG 8000 + sodium sulfate) and (PEG 8000 + magnesium sulfate) aqueous two-phase system: Relative hydrophobicity of the equilibrium phases. Journal of Chemical Thermodynamics, 91, 321-326, 2015
A.P. Carneiro, O. Rodríguez, E.A. Macedo. Separation of carbohydrates and sugar alcohols from ionic liquids using antisolvents. Separation and Purification Technology, 132, 496-504, 2014
S.C. Silvério, O. Rodríguez, A.P.M. Tavares, J.A. Teixeira, E.A. MacEdo. Laccase recovery with aqueous two-phase systems: Enzyme partitioning and stability. Journal of Molecular Catalysis B: Enzymatic, 87, 37-43, 2013
F.M. Maia, O. Rodríguez, E.A. Macedo. Delta G (CH2) in biphasic systems of water and bis(trifluoromethylsulfonyl)imide-based ionic liquids. Journal of Chemical and Engineering Data, 58, 1565-1570, 2013
A.P. Carneiro, O. Rodríguez, E.A. Macedo. Fructose and glucose dissolution in ionic liquids: Solubility and thermodynamic modeling. Industrial and Engineering Chemistry Research, 52, 3424-3435, 2013
Faculdade de Engenharia da Universidade do Porto
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